NMR-Based Metabolomic Profiling of Urine: Evaluation for Application in Prostate Cancer Detection

Archived Abstract of Former PSC Researcher

MacKinnon, Neil, Trivellore Raghunathan, Wencheng Ge, Peisong Han, Javed Siddiqui, John T. Wei, Arul M. Chinnaiyan, Thekkelnaycke M. Rajendiran, an, et al. 2019. "NMR-Based Metabolomic Profiling of Urine: Evaluation for Application in Prostate Cancer Detection." Natural Product Communications, 14(5).

Detection of prostate cancer (PCa) and distinguishing indolent versus aggressive forms of the disease is a critical clinical challenge. The current clinical test is circulating prostate-specific antigen levels, which faces particular challenges in cancer diagnosis in the range of 4 to 10 ng/mL. Thus, a concerted effort toward building a noninvasive biomarker panel has developed. In this report, the hypothesis that nuclear magnetic resonance (NMR)-derived metabolomic profiles measured in the urine of biopsy-negative versus biopsy-positive individuals would nominate a selection of potential biomarker signals was investigated. 1H NMR spectra of urine samples from 317 individuals (111 biopsy-negative, 206 biopsy-positive) were analyzed. A double cross-validation partial least squares-discriminant analysis modeling technique was utilized to nominate signals capable of distinguishing the two classes. It was observed that after variable selection protocols were applied, a subset of 29 variables produced an area under the curve (AUC) value of 0.94 after logistic regression analysis, whereas a "master list" of 18 variables produced a receiver operating characteristic ROC) AUC of 0.80. As proof of principle, this study demonstrates the utility of NMR-based metabolomic profiling of urine biospecimens in the nomination of PCa-specific biomarker signals and suggests that further investigation is certainly warranted.


Methodology Population Health

Browse | Search | Next

PSC In The News

RSS Feed icon

Erin Cech explains her research on the "passion principle," and how America's obsession with pursuing and pushing towards a "#dreamjob" is flawed

Shaefer notes success of initial CARES Act stimulus and concerns over a new round of COVID-19 shutdown support under a Biden presidency

More News


Faul's three-nation research to examine relationships between social factors and epigenetics

Open for Registration: Principles of Text Analysis Workshop

More Highlights

Connect with PSC follow PSC on Twitter Like PSC on Facebook